Cytokine profile of chronic sinusitis in patients with cystic fibrosis

Background: The inflammatory-cell and cytokine profiles of chronic sinusitis (CS) are well documented in the literature. In contrast, little is known about the pathogenesis of this condition in patients with cystic fibrosis (CF). Objective: To determine whether patients with CF have inflammatory-cell and cytokine profiles that differ from other patients with CS. Methods: Patients with CF (n=7) and adults with CS (n=7) undergoing functional endoscopic sinus surgery were recruited for the study. Patients with no allergies or sinus disease (n=6) were used as controls. Using immunohistochemical-analysis, we assessed sinus mucosal specimens for the presence of T lymphocytes, eosinophils, macrophages, and neutrophils. Using in situ hybridization, we assessed the expression of interleukin (IL) 4, IL-5, IL-8, IL-10, and interferon gamma. Results: There was a higher number of neutrophils, macrophages, and cells expressing messenger RNA for interferon gamma and IL-8 in patients with CF than in patients with CS or in controls (P<.01). The number of eosinophils and cells expressing messenger RNA for IL-4, IL-5, and IL-10 was higher inpatients with CS than in those with CF and controls (P<.01). Conclusions: Sinus disease in patients with CF presents different inflammatory-cell and cytokine profiles than that seen in other patients with CS. These results may explain the difference in response to treatment in the CF group.