Effective bone engineering with periosteum-derived cells

被引:97
作者
Agata, H.
Asahina, I.
Yamazaki, Y.
Uchida, M.
Shinohara, Y.
Honda, M. J.
Kagami, H.
Ueda, M.
机构
[1] Univ Tokyo, Inst Med Sci, Div Stem Cell Engn, Tokyo, Japan
[2] Nagoya Univ, Dept Oral & Maxillofacial Surg, Grad Sch Med, Nagoya, Aichi, Japan
[3] Nagasaki Univ, Grad Sch Biomed Sci, Dept Regenerat Oral Surg, Nagasaki 852, Japan
[4] Kitasato Univ, Sch Med, Dept Plast & Reconstruct Surg, Kanagawa, Japan
关键词
marrow stromal cells; periosteal cells; osteogenic potential; bFGF; BMP-2;
D O I
10.1177/154405910708600113
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Bone augmentation via tissue engineering has generated significant interest. We hypothesized that periosteum-derived cells could be used in place of bone marrow stromal cells (which are widely used) in bone engineering, but the differences in osteogenic potential between these 2 cell types are unclear. Here, we compared the osteogenic potential of these cells, and investigated the optimal osteoinductive conditions for periosteum- derived cells. Both cell types were induced, via bFGF and BMP-2, to differentiate into osteoblasts. Periosteal cells proliferated faster than marrow stromal cells, and osteogenic markers indicated that bone marrow stromal cells were more osteogenic than periosteal cells. However, pre-treatment with bFGF made periosteal cells more sensitive to BMP-2 and more osteogenic. Transplants of periosteal cells treated with BMP-2 after pre-treatment with bFGF formed more new bone than did marrow stromal cells. Analysis of these data suggests that combined treatment with bFGF and BMP-2 can make periosteum a highly useful source of bone regeneration.
引用
收藏
页码:79 / 83
页数:5
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