Differential regulation of p53 function by protein kinase C isoforms revealed by a yeast cell system

被引：20

作者：

Coutinho, Isabel ^{[1
]}

Pereira, Gil ^{[1
]}

Leao, Mariana ^{[1
]}

Goncalves, Jorge ^{[2
]}

Corte-Real, Manuela ^{[3
]}

Saraiva, Lucilia ^{[1
]}

机构：

[1] Univ Porto, Fac Farm, REQUIMTE, Microbiol Lab, P-4050047 Oporto, Portugal

[2] Univ Porto, Fac Farm, REQUIMTE, Farmacol Lab, P-4050047 Oporto, Portugal

[3] Univ Minho, Ctr Biol Mol & Ambiental, P-4710057 Braga, Portugal

来源：

FEBS LETTERS | 2009年 / 583卷 / 22期

关键词：

p53; Protein kinase C isoform; Cell growth; Cell cycle; phosphorylation; Yeast; SACCHAROMYCES-CEREVISIAE; TUMOR-SUPPRESSOR; APOPTOSIS; PHOSPHORYLATION; ACTIVATION;

D O I：

10.1016/j.febslet.2009.10.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The complexity of the mammalian p53 pathway and protein kinase C (PKC) family has hampered the discrimination of the effect of PKC isoforms on p53 activity. Using yeasts co-expressing the human wild-type p53 and a mammalian PKC-alpha, -delta, -epsilon or -zeta, we showed a differential regulation of p53 activity and phosphorylation state by PKC isoforms. Whereas PKC-alpha reduced the p53-induced yeast growth inhibition and cell cycle arrest, PKC-delta and -epsilon enhanced the p53 activity through p53 phosphorylation, and PKC-zeta had no effect on p53. This work identified positive and negative p53 regulators which represent promising pharmacological targets in anti-cancer therapy. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：3582 / 3588

页数：7

共 15 条

[1]

Human p53 induces cell death and downregulates thioredoxin expression in Saccharomyces cerevisiae [J].