The IrrE protein of Deinococcus radiodurans R1 is a novel regulator of recA expression

被引:161
作者
Earl, AM
Mohundro, MM
Mian, IS
Battista, JR
机构
[1] Louisiana State Univ & A&M Coll, Dept Biol Sci, Baton Rouge, LA 70803 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA
关键词
D O I
10.1128/JB.184.22.6216-6224.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
IRS24 is a DNA damage-sensitive strain of Deinococcus radiodurans strain 302 carrying a mutation in an uncharacterized locus designated irrE. Five overlapping cosmids capable of restoring ionizing radiation resistance to IRS24 were isolated from a genomic library. The ends of each cloned insert were sequenced, and these sequences were used to localize irrE to a 970-bp region on chromosome I of D. radiodurans R1. The irrE gene corresponds to coding sequence DR0167 in the RI genome. The irrE gene encodes a 35,000-Da protein that has no similarity to any previously characterized peptide. The irrE locus of R1 was also inactivated by transposon mutagenesis, and this strain was sensitive to ionizing radiation, UV light, and mitomycin C. Preliminary findings indicate that IrrE is a novel regulatory protein that stimulates transcription of the recA gene following exposure to ionizing radiation.
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页码:6216 / 6224
页数:9
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