Improved cell growth and total flavonoids of Saussurea medusa on solid culture medium supplemented with rare earth elements

被引：34

作者：

Yuan, XF ^{[1
]}

Wang, Q ^{[1
]}

Zhao, B ^{[1
]}

Wang, YC ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100080, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 22期

基金：

中国国家自然科学基金;

关键词：

callus; rare earth element; Saussurea medusa; total flavonoids;

D O I：

10.1023/A:1020931608159

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Callus cultures of Saussurea medusa were cultivated on solid culture medium supplemented with either Ce3+, La3+,Nd3+ or a mixture of rare earth elements. Ce3+, 0.05 mM, gave the highest biomass (0.53 g dry wt per flask) and total flavonoids (27.5 mg per flask), which were, 70% and 100% higher than those without Ce3+ addition, respectively. Ce3+, 0.01-0.1 mM, or La3+, 0.05 mM, or the mixture of rare earth elements, 0.025-0.1 mM, can substitute for 6-benzyladenine, and 0. 025 mM Ce3+ can partly substitute for naphthaleneacetic acid in promoting cell growth and biosynthesis of total flavonoids in S. medusa.

引用

页码：1889 / 1892

页数：4

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