Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family

被引：81

作者：

Luo, Cong

Zhu, Yongliang

Jiang, Tiejun

Lu, Xiaoyong

Zhang, Wo

Jing, Qifeng

Li, Jia

Pang, Linrong

Chen, Kangjie

Qiu, Fuming

Yu, Xiuyan

Yang, Jiahui

Huang, Jian ^{[1
]}

机构：

[1] Zhejiang Univ, Affiliatd Hosp 2, Sch Med, Inst Canc, Hangzhou 310009, Peoples R China

[2] Zhejiang Univ, Affiliatd Hosp 2, Sch Med, Dept Gastroenterol, Hangzhou 310009, Peoples R China

来源：

TOXICOLOGY | 2007年 / 229卷 / 03期

基金：

中国国家自然科学基金;

关键词：

matrine; apoptosis; Bcl-2; family;

D O I：

10.1016/j.tox.2006.10.020

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：245 / 252

页数：8

共 25 条

[1]

Proapoptotic Bcl-2 relative bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity [J].