Enhanced antisense effects resulting from an improved streptolysin-O protocol for oligodeoxynucleotide delivery into human leukaemia cells.

被引：23

作者：

Giles, RV

Grzybowski, J

Spiller, DG

Tidd, DM

机构：

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 7-9期

关键词：

D O I：

10.1080/07328319708006151

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved protocol for delivering oligodeoxynucleotides into the cytoplasm and nucleus of human cells in culture using streptolysin O is described. The new procedure permitted reduced target protein expression to result from antisense suppression of target mRNA levels.

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页码：1155 / 1163

页数：9

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