Isolation and sequence analysis of a small cryptic plasmid pRK10 from a corrosion inhibitor degrading strain Serratia marcescens ACE2

被引：9

作者：

Ibrahim, Kalibulla Syed ^{[1
]}

Bakkiyaraj, Dhamodharan ^{[1
]}

James, Roswin ^{[1
]}

Babu, Thambidurai Ganesh ^{[1
]}

Pandian, Shunmugiah Thevar Karutha ^{[1
]}

机构：

[1] Alagappa Univ, Dept Biotechnol, Karaikkudi 630003, Tamil Nadu, India

来源：

PLASMID | 2009年 / 62卷 / 03期

关键词：

Serratia marcescens; Plasmid sequence; Plasmid mobilization; Cryptic plasmid; COMPLETE NUCLEOTIDE-SEQUENCE; DNA-SEQUENCE; RNA-I; RESISTANCE; SITE; MOBILIZATION; RECOMBINATION; REPLICATION; ALIGNMENT; HOMOLOGY;

D O I：

10.1016/j.plasmid.2009.08.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学];

摘要：

The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens; in sizes of over 70 kb, pRK10 is only 4241 bp long with 53% G + C content and has five coding sequences representing a coding percentage of 65.41. This small plasmid consists of one Tdh gene, four mobilization genes, mobCABD, and an origin of replication homologous to those of CoIE1-type plasmids. Analysis of the five open reading frames identified on the plasmid suggests the presence of genes involved in replication and mobilization containing sequences homologous to the bom region and mobCABD genes of ColE1 and Tdh from Acinetobacter baumannii str. AYE. Results also indicate that pRK10 does not encode any gene for antibiotic/heavy metal resistance. Copy number and incompatibility of the plasmid with plasmids of ColE1 origin of replication was determined and it is quite stable in its natural host as well as in Escherichia coli DH5 alpha. This relatively small plasmid will be useful for construction of shuttle vectors to facilitate the genetic analysis. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：183 / 190

页数：8

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