Purification, characterization, and biological compartmentalization of rat fetal antigen 1

被引：6

作者：

Carlsson, HE

Persdotter-Hedlund, G

Fries, E

Eriksson, UJ

Hau, J

机构：

[1] Uppsala Univ, Dept Physiol, Div Comparat Med, SE-75123 Uppsala, Sweden

[2] Uppsala Univ, Dept Med Biochem & Microbiol, Sect Med Biochem, SE-75123 Uppsala, Sweden

[3] Uppsala Univ, Dept Med Cell Biol, SE-75123 Uppsala, Sweden

来源：

BIOLOGY OF REPRODUCTION | 2000年 / 63卷 / 01期

关键词：

conceptus; developmental biology; pregnancy;

D O I：

10.1095/biolreprod63.1.30

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

This study has established the rat as an animal model for the analysis of the biological role of fetal antigen 1 (FA1), a protein previously described in humans and mice. FA1 was purified from rat amniotic fluid by immunospecific affinity chromatography. Immunochemical identity between mouse and rat FA1 was established by crossed tandem immunoelectrophoresis. Molecular size was analyzed by mass spectrometry (33 kDa). The amino acid composition was determined, and the amino acid sequence was analyzed. The overall amino acid composition and sequence of the 28 first N-terminal amino acids were identical to the corresponding parts of rat preadipocyte factor 1 and rat adrenal zone glomerulosa protein. Extensive sequence similarity was found between rat and mouse FA1 (86%) and between rat and human FA1 (82%). The concentration of FA1 in fetal serum, maternal serum, urine, and amniotic fluid in rats was determined using an ELISA. The highest concentrations were found in fetal serum and amniotic fluid around Day 18 of pregnancy. This is the first report on the physicochemical characteristics and compartmentalization of rat FA1.

引用

页码：30 / 33

页数：4

共 16 条

[1]

Bachmann E, 1996, J REPROD FERTIL, V107, P279

[2] Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/Delta-like protein in pancreatic islets: Molecular cloning and expression pattern during development and growth of the endocrine pancreas [J].