Identification of Ruscus steroidal saponins by HPLC-MS analysis

被引：35

作者：

de Combarieu, E ^{[1
]}

Falzoni, M ^{[1
]}

Fuzzati, N ^{[1
]}

Gattesco, F ^{[1
]}

Giori, A ^{[1
]}

Lovati, M ^{[1
]}

Pace, R ^{[1
]}

机构：

[1] Indena SPA, Res & Dev Labs, I-20090 Settala, MI, Italy

来源：

FITOTERAPIA | 2002年 / 73卷 / 7-8期

关键词：

Ruscus aculeatus; Ruscus hypoglossum; Ruscus colchicus; ruscoside; ruscin; ruscogenin; neoruscogenin; HPLC-UV; HPLC-ESI-MS; 1-O-[alpha-L-rhamnopyranosyl-(1; 2)-6-O-acetyl-beta-D-galactopyranosyl]-1; beta; 3; 22; xi; 26-tetrahydroxy-furost-5(6)-en-26-O-beta-D-glucopyranoside;

D O I：

10.1016/S0367-326X(02)00220-4

中图分类号：

R914 [药物化学];

学科分类号：

100701 [药物化学];

摘要：

A novel HPLC-UV method has been developed for the fingerprint analysis of the steroidal saponins in the rhizomes of three Ruscus species (Ruscus aculeatus, Ruscus hypoglossum and Ruscus colchicus). Saponins were identified by HPLC-ESI-MS. During the study a new major saponin was detected in the rhizomes of R. hypoglossum and R. colchicus. The structure of the new compound was defined as 1-O-[alpha-L-rhamnopyranosyl-(1-->2)-6-O-acetyl-beta-D-galactopyranosyl]-1beta,3beta,22xi,26-tetrahydroxy-furost-5(6)-en-26-O-beta-D-glucopyranoside (8) by spectral analysis. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：583 / 596

页数：14

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