Analysis of inositol phosphates in heart tissue using anion-exchange high-performance liquid chromatography

被引：20

作者：

Woodcock, EA

机构：

[1] Cellular Biochemistry Laboratory, Baker Medical Research Institute, Prahran

[2] Baker Medical Research Institute, Commercial Road, Prahran

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 1997年 / 172卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

inositol phosphates; anion exchange HPLC; heart;

D O I：

10.1023/A:1006871722652

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The pathways of release and metabolism of inositol phosphates in intact heart tissue are different from those observed in isolated cardiomyocytes in culture. Thus, it is essential that methods are available for the quantitation of inositol phosphates in intact tissue preparations. This manuscript describes methods which allow the quantitation of inositol phosphates in different heart preparations including isolated atria and intact perfused heart. The availability of such methods should facilitate study of the role of inositol phosphates in cardiac control mechanisms under physiological and pathological conditions.

引用

页码：121 / 127

页数：7

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[1] THE NOREPINEPHRINE-STIMULATED INOSITOL PHOSPHATE RESPONSE IN HUMAN ATRIA [J].