DNA sensor for the determination of antitumor platinum compounds

被引：72

作者：

Brabec, V ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic

来源：

ELECTROCHIMICA ACTA | 2000年 / 45卷 / 18期

关键词：

DNA; biosensor; platinum; cancer; graphite electrode;

D O I：

10.1016/S0013-4686(00)00370-4

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A wax impregnated graphite electrode coated by linearized plasmid DNA is employed as a sensitive biosensor for the detection of platinum compounds. The sensor relies on monitoring changes in the intrinsic electrooxidation response of the surface-confined DNA resulting from its interaction with platinum compounds and requires no label or indicator. Short reaction times (2-10 min) are sufficient for monitoring submicromolar levels of platinum complexes. It has been suggested that DNA biosensors could be used for quantitating antitumor platinum drugs in various samples including those used when studying mechanisms underlying their antitumor effectiveness. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：2929 / 2932

页数：4

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