Transport and consumption rate of O2 in alginate gel beads entrapping hepatocytes

被引：7

作者：

Annesini, MC

Castelli, G

Conti, F

De Virgiliis, LC

Marrelli, L ^{[1
]}

Miccheli, A

Satori, E

机构：

[1] Univ Rome La Sapienza, Dept Chem Engn, Rome, Italy

[2] Univ Rome La Sapienza, Dept Chem, I-00185 Rome, Italy

[3] Univ Aquila, Dept Basic & Appl Biol, I-67100 Laquila, Italy

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 10期

关键词：

alginate; bioartificial liver; hepatocytes;

D O I：

10.1023/A:1005623732252

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Experiments carried out with hepatocytes entrapped in alginate gel particles with a cell density of about 10(7) cells ml(-1) showed an oxygen consumption rate of about 2.2x10(-16) mol cell(-1) s(-1). Under these conditions, no inner anoxic core is present in 1.8 mm diameter beads. From these data, the maximum bead size consistent with non-critical O-2 concentration at the bead center has been evaluated for different cell densities and O-2 concentrations in the medium.

引用

页码：865 / 870

页数：6

共 22 条

[1]

The dependence of hepatic function upon sufficient oxygen supply during prolonged isolated rat liver perfusion [J].