Fluorescence-based Sensing of 2,4,6-Trinitrotoluene (TNT) Using a Multi-channeled Poly(methyl methacrylate) (PMMA) Microimmunosensor

被引：21

作者：

Charles, Paul T. ^{[1
]}

Adams, Andre A. ^{[1
]}

Howell, Peter B., Jr. ^{[1
]}

Trammell, Scott A. ^{[1
]}

Deschamps, Jeffrey R. ^{[1
]}

Kusterbeck, Anne W. ^{[1
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

来源：

SENSORS | 2010年 / 10卷 / 01期

关键词：

TNT; immunosensor; fluorescence; PMMA; microchannel; antibody; TRACE-LEVEL DETECTION; IMMUNOSENSOR; FABRICATION; IMMUNOASSAY; EXPLOSIVES; PRODUCTS;

D O I：

10.3390/s100100876

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescence immunoassays employing monoclonal antibodies directed against the explosive 2,4,6-trinitrotoluene (TNT) were conducted in a multi-channel microimmunosensor. The multi-channel microimmunosensor was prepared in poly (methyl methacrylate) (PMMA) via hot embossing from a brass molding tool. The multi-channeled microfluidic device was sol-gel coated to generate a siloxane surface that provided a scaffold for antibody immobilization. AlexaFluor-cadaverine-trinitrobenzene (AlexaFluor-Cad- TNB) was used as the reporter molecule in a displacement immunoassay. The limit of detection was 1-10 ng/mL (ppb) with a linear dynamic range that covered three orders of magnitude. In addition, antibody crossreactivity was investigated using hexahydro-1,3,5-triazine (RDX), HMX, 2,4-dinitrotoluene (DNT), 4-nitrotoluene (4-NT) and 2-amino-4,6-DNT.

引用

页码：876 / 889

页数：14

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