Epitopes of human leukocyte antigen class I antibodies found in sera of normal healthy males and cord blood

被引:122
作者
El-Awar, Nadim [1 ]
Terasaki, Paul I. [2 ]
Nguyen, Anh [1 ]
Sasaki, Nori [1 ]
Morales-Buenrostro, Luis E. [3 ]
Saji, Hiroh [4 ]
Maruya, Etsuko [4 ]
Poli, Francesca [5 ]
机构
[1] One Lambda Inc, Canoga Pk, CA 90064 USA
[2] Terasaki Fdn Lab, Los Angeles, CA 90064 USA
[3] Natl Inst Med Sci & Nutr Salvador Zubiran, Dept Nephrol & Mineral Metab, Mexico City 14000, DF, Mexico
[4] HLA Lab NPO, Kyoto 6068396, Japan
[5] Osped Maggiore Policlin, IRCCS Fdn, Dept Regenerat Med, N Italy Reference Ctr, Milan, Italy
关键词
Epitopes of natural HLA antibodies; Recombinant HLA class I antigens; Cryptic epitopes; Exposed epitopes; Single antigen; 3-DIMENSIONAL STRUCTURE; HLA; COMPLEX;
D O I
10.1016/j.humimm.2009.06.020
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
This study defines 96 epitopes targeted by human leukocyte antigen (HLA) antibodies reported in the sera of normal healthy males with no history of deliberate alloimmunizations and in cord blood. These epitopes are accessible for antibody binding on either the intact or the dissociated forms of recombinant HLA class I single antigens. Sixty percent of the epitopes are accessible on dissociated antigens, are defined mostly by hidden amino acids, and are designated as cryptic epitopes. All 96 epitopes are located exclusively on A-, B-, or C-locus antigens except for one interlocus epitope. All sera in this study were tested in parallel, using single antigen beads that bear either intact or dissociated HLA antigens and antibodies with nearly identical specificities were identified in all tested sera. Because the specificities of these naturally occurring antibodies are unavoidably detected when testing for specificities of alloantibodies, it may be necessary to clearly differentiate the two forms of antibody. To date, the relevance of these antibodies in transplantation is unknown, but even if they are determined to be irrelevant to graft rejection, awareness of the newly identified epitopes could prove useful in avoiding the unnecessary exclusion of potential transplant donors. (C) 2009 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.
引用
收藏
页码:844 / 853
页数:10
相关论文
共 11 条
[1]   3-DIMENSIONAL STRUCTURE OF AN ANTIGEN-ANTIBODY COMPLEX AT 2.8-A RESOLUTION [J].
AMIT, AG ;
MARIUZZA, RA ;
PHILLIPS, SEV ;
POLJAK, RJ .
SCIENCE, 1986, 233 (4765) :747-753
[2]   Intact HLA Not β2m-free Heavy Chain-Specific HLA Class I Antibodies Are Predictive of Graft Failure [J].
Cai, Junchao ;
Terasaki, Paul I. ;
Anderson, Naomi ;
Lachmann, Nils ;
Schoenemann, Constanze .
TRANSPLANTATION, 2009, 88 (02) :226-230
[3]  
COLLINS ZV, 1973, TISSUE ANTIGENS, V3, P358
[4]   HLA class I epitopes: Recognition of binding sites by mAbs or eluted alloantibody confirmed with single recombinant antigens [J].
El-Awar, Nadim ;
Lee, Jar-How ;
Tarsitani, Christine ;
Terasaki, Paul I. .
HUMAN IMMUNOLOGY, 2007, 68 (03) :170-180
[5]   Human leukocyte antigen class I epitopes: Update to 103 total epitopes, including the C locus [J].
El-Awar, Nadim R. ;
Akaza, Tatsuya ;
Terasaki, Paul I. ;
Nguyen, Anh .
TRANSPLANTATION, 2007, 84 (04) :532-540
[6]  
IDICA A, 2006, CLIN TRANSPLANTS TER, V161
[7]  
LEPAGE V, 1976, TISSUE ANTIGENS, V8, P139
[8]   Natural Human Leukocyte Antigen Antibodies Found in Nonalloimmunized Healthy Males [J].
Morales-Buenrostro, Luis E. ;
Terasaki, Paul I. ;
Marino-Vazquez, Lluvia A. ;
Lee, Jar-How ;
El-Awar, Nadim ;
Alberu, Josefina .
TRANSPLANTATION, 2008, 86 (08) :1111-1115
[9]   Single human leukocyte antigen flow cytometry beads for accurate identification of human leukocyte antigen antibody specificities [J].
Pei, R ;
Lee, JH ;
Shih, NJ ;
Chen, M ;
Terasaki, PI .
TRANSPLANTATION, 2003, 75 (01) :43-49
[10]   3-DIMENSIONAL STRUCTURE OF AN ANTIBODY-ANTIGEN COMPLEX [J].
SHERIFF, S ;
SILVERTON, EW ;
PADLAN, EA ;
COHEN, GH ;
SMITHGILL, SJ ;
FINZEL, BC ;
DAVIES, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (22) :8075-8079