Characterization and analysis of a new gene involved in glucose starvation response in Enterococcus faecalis

被引：5

作者：

Capiaux, H ^{[1
]}

Giard, JC ^{[1
]}

Lemarinier, S ^{[1
]}

Auffray, Y ^{[1
]}

机构：

[1] Univ Caen, Inst Rech Biol Appl, Lab Microbiol Environm, F-14032 Caen, France

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2000年 / 55卷 / 1-3期

关键词：

Enterococcus faecalis; environmental stresses; general stress protein;

D O I：

10.1016/S0168-1605(00)00183-5

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The genome sequence, of Enterococcus faecalis, led us to discover that gls24 encoding a general stress protein, seems to be in the second last position of a putative six-gene operon structure. Interestingly, another gene named orf4 located just upstream from gls24 shows strong identity (72%) with this last. To determine the role of the orf4 gene in E. faecalis, we have constructed a mutant strain by homologous recombination. Phenotypic analysis of these cells, reveals that Orf4 is probably involved in cell structure in stationary phase. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：99 / 102

页数：4

共 3 条

[1] CLONING, EXPRESSION, SEQUENCE-ANALYSIS, AND SITE-DIRECTED MUTAGENESIS OF THE TN5306-ENCODED N-5-(CARBOXYETHYL)ORNITHINE SYNTHASE FROM LACTOCOCCUS-LACTIS K1 [J].

DONKERSLOOT, JA ;

THOMPSON, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (20) :12226-12234

[2] Glucose starvation response in Enterococcus faecalis JH2-2: Survival and protein analysis [J].

Giard, JC ;

Hartke, A ;

Flahaut, S ;

Boutibonnes, P ;

Auffray, Y .

RESEARCH IN MICROBIOLOGY, 1997, 148 (01) :27-35

[3]

Hartke A, 1998, APPL ENVIRON MICROB, V64, P4238

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