Monitoring of active HHV-6 infection in bone marrow transplant recipients by real time PCR; Comparison to detection of viral DNA in plasma by qualitative PCR

被引：22

作者：

Ihira, M

Yoshikawa, T ^{[1
]}

Suzuki, K

Ohashi, M

Suga, S

Horibe, K

Tanaka, N

Kimura, H

Kojima, S

Kato, K

Matsuyama, T

Nishiyama, Y

Asano, Y

机构：

[1] Nagoya Univ, Sch Med, Virol Lab, Res Inst Dis Metab & Control, Nagoya, Aichi 4668550, Japan

[2] Fujita Hlth Univ, Sch Med, Dept Pediat, Toyoake, Aichi 4701192, Japan

[3] Nagoya Univ, Sch Med, Dept Pediat, Nagoya, Aichi 466, Japan

[4] Japanese Red Cross Nagoya First Hosp, Childrens Med Ctr, Div Hematol Oncol, Nagoya, Aichi 4530046, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2002年 / 46卷 / 10期

关键词：

HHV-6; real time PCR; plasma PCR; bone marrow transplantation;

D O I：

10.1111/j.1348-0421.2002.tb02754.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Twelve (46%) of the 26 patients had human herpesvirus 6 (HHV-6) viremia after bone marrow transplant (BMT). All isolates were recovered from the samples obtained at 2 weeks after BMT. The sensitivity and the specificity of detection of viral DNA in plasma by qualitative polymerase chain reaction (PCR) for monitoring active virus replication were 92% and 97% respectively. Moreover, the positive (85%) and negative (99%) predictive values were also high. The patients with HHV-6 viremia showed a clear peak in HHV-6 DNA in peripheral blood mononuclear cells (PBMCs) at 2 weeks after BMT, which was measured by real time PCR. The virus DNA level in PBMCs between the two groups (patients with viremia and patients without viremia) was statistically different at 2 weeks after BMT (P = 0.033). In patients with HHV-6 viremia, mean HHV-6 DNA copy number was higher in the samples collected at 2 weeks after BMT than the samples collected at any other time period.

引用

页码：701 / 705

页数：5

共 27 条

[1] VIREMIA AND NEUTRALIZING ANTIBODY-RESPONSE IN INFANTS WITH EXANTHEM SUBITUM [J].