Modified high-performance liquid chromatographic method for the determination of ertapenem in human urine: enhanced selectivity and automation

被引：15

作者：

Musson, DG ^{[1
]}

Kitchen, CJ ^{[1
]}

Hsieh, JYK ^{[1
]}

Birk, KL ^{[1
]}

机构：

[1] Merck Res Labs, W Point, PA 19486 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 779卷 / 02期

关键词：

ertapenem;

D O I：

10.1016/S1570-0232(02)00377-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A column-switching, reversed-phase high-performance liquid chromatographic (HPLC) assay for a new structurally unique carbapenem antibiotic, ertapenem, in urine has been improved for selectivity and automated using a Packard MultiPROBE(R) II EX pipetting station. The method uses column-switching for on-line extraction of the urine sample. The extraction column, analytical column, mobile phase, and timing of the column-switching valve have been changed to enhance selectivity for the analyte over endogenous background material. Sample transfer and dilution prior to direct-injection into the HPLC system have been accomplished using a Packard MultiPROBE(R) II EX robotic liquid handling system. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：341 / 346

页数：6

共 3 条

[1]

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[2]

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Majumdar, AK ;

Rogers, JD .

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[3]

Preparation, isolation, and characterization of dimeric degradation products of the 1β-methylcarbapenem antibiotic, ertapenem [J].

Sajonz, P ;

Natishan, TK ;

Wu, Y ;

Williams, JM ;

Pipik, B ;

DiMichele, L ;

Novak, T ;

Pitzenberger, S ;

Dubost, D ;

Almarsson, Ö .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2001, 24 (19) :2999-3015

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