Structural organization of the RNA polymerase-promoter open complex

被引：159

作者：

Naryshkin, N ^{[1
]}

Revyakin, A ^{[1
]}

Kim, YG ^{[1
]}

Mekler, V ^{[1
]}

Ebright, RH ^{[1
]}

机构：

[1] Rutgers State Univ, Waksman Inst, Howard Hughes Med Inst, Dept Chem, Piscataway, NJ 08854 USA

来源：

CELL | 2000年 / 101卷 / 06期

关键词：

D O I：

10.1016/S0092-8674(00)80872-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.

引用

页码：601 / 611

页数：11

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