Enhanced spectral resolution in immobilized peptides and proteins by combining chemical shift sum and difference spectroscopy

被引：15

作者：

Luca, S ^{[1
]}

Baldus, M ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, Dept NMR Based Struct Biol, D-37077 Gottingen, Germany

来源：

JOURNAL OF MAGNETIC RESONANCE | 2002年 / 159卷 / 02期

关键词：

chemical shifts; correlation spectroscope; magic angle spinning; protein backbone structure; spectral resolution;

D O I：

10.1016/S1090-7807(02)00019-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A two-dimensional correlation experiment is introduced that records the sum and difference chemical shift of two scalar or dipolar coupled nuclei. Statistical results indicate that the suggested pulse scheme can significantly increase the possibility of separating chemical shift contributions due to residue type and backbone conformation in immobilized peptides and proteins. Experimental applications demonstrate the theoretical concept and lead to the predicted resolution enhancement between different amino acid types and among protein residues of different secondary structure. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：243 / 249

页数：7

共 45 条

[1] NUCLEAR MAGNETIC RESONANCE SPECTRA FROM A CRYSTAL ROTATED AT HIGH SPEED [J].

ANDREW, ER ;

BRADBURY, A ;

EADES, RG .

NATURE, 1958, 182 (4650) :1659-1659

[2] Triple resonance solid state NMR experiments with reduced dimensionality evolution periods [J].

Astrof, NS ;

Lyon, CE ;

Griffin, RG .

JOURNAL OF MAGNETIC RESONANCE, 2001, 152 (02) :303-307

[3] Total correlation spectroscopy in the solid state. The use of scalar couplings to determine the through-bond connectivity [J].

Baldus, M ;

Meier, BH .

JOURNAL OF MAGNETIC RESONANCE SERIES A, 1996, 121 (01) :65-69

[4] Correlation experiments for assignment and structure elucidation of immobilized polypeptides under magic angle spinning [J].

Baldus, M .

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2002, 41 (1-2) :1-47

[5] NATURAL ABUNDANCE C-13-C-13 COUPLING OBSERVED VIA DOUBLE-QUANTUM COHERENCE [J].

BAX, A ;

FREEMAN, R ;

KEMPSELL, SP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1980, 102 (14) :4849-4851

[6] HETERONUCLEAR DECOUPLING IN ROTATING SOLIDS [J].

BENNETT, AE ;

RIENSTRA, CM ;

AUGER, M ;

LAKSHMI, KV ;

GRIFFIN, RG .

JOURNAL OF CHEMICAL PHYSICS, 1995, 103 (16) :6951-6958

[7] ON THE INTERACTION OF NUCLEAR SPINS IN A CRYSTALLINE LATTICE [J].

BLOEMBERGEN, N .

PHYSICA, 1949, 15 (3-4) :386-426

[8] MULTIPLE QUANTUM SPIN-ECHO SPECTROSCOPY [J].

BODENHAUSEN, G ;

VOLD, RL ;

VOLD, RR .

JOURNAL OF MAGNETIC RESONANCE, 1980, 37 (01) :93-106

[9] Validation of protein structure from anisotropic carbonyl chemical shifts in a dilute liquid crystalline phase [J].

Cornilescu, G ;

Marquardt, JL ;

Ottiger, M ;

Bax, A .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (27) :6836-6837

[10] SECONDARY AND TERTIARY STRUCTURAL EFFECTS ON PROTEIN NMR CHEMICAL-SHIFTS - AN ABINITIO APPROACH [J].

DEDIOS, AC ;

PEARSON, JG ;

OLDFIELD, E .

SCIENCE, 1993, 260 (5113) :1491-1496

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