Depressant effects on inspiratory and expiratory activity produced by chemical activation of Botzinger complex neurons in the rabbit

The respiratory role of the Botzinger complex (Bot. c.) was investigated in alpha-chloralose-urethane or pentobarbitone anesthetized rabbits by means of microinjections of DL-homocysteic acid (DLH). The animals were either spontaneously breathing or vagotomized, paralysed and artificially ventilated. Both phrenic and abdominal activities were monitored; extracellular recordings from medullary respiration-related neurons were performed. Unilateral microinjections (5-30 nl) of DLH (160 mM) into the Bot. c., at sites where intense expiratory activity with an augmenting discharge pattern was encountered, provoked mild or moderate depressant effects on inspiratory activity characterized by decreases in frequency as well as in peak amplitude and rate of rise of phrenic nerve discharge. Stronger depressant effects up to complete apnea were consistently obtained in response to bilateral microinjections. Concomitant depressant effects on the activity of both expiratory motoneurons and expiration-related (ER) neurons of the caudal ventral respiratory group (cVRG) were observed. At variance with previous findings in the cat, the results indicate that chemical activation of Bot. c. augmenting ER neurons may exert inhibitory influences not only on inspiratory activity, but also on cVRG ER neurons and, hence, on expiratory motoneurons. The functional role of the Bot. c. in the control of respiration deserves further investigations; present findings suggest that the rabbit may profitably be used for such a purpose.