Folding and aggregation of export-defective mutants of the maltose-binding protein

被引：8

作者：

Betton, JM ^{[1
]}

Phichith, D ^{[1
]}

Hunke, S ^{[1
]}

机构：

[1] Inst Pasteur, CNRS URA 2185, Unite Repliement & Modelisat Prot, F-75724 Paris 15, France

来源：

RESEARCH IN MICROBIOLOGY | 2002年 / 153卷 / 07期

关键词：

heat-shock proteins; maltose; protein folding; protein precursors;

D O I：

10.1016/S0923-2508(02)01338-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We previously characterized a defective-folding variant of the periplasmic maltose-binding protein, MalE31. To examine the alternative folding pathways open to the MalE31 precursor, we have analyzed the cellular fates of this aggregation-prone protein carrying altered signal sequences. Our results are most easily interpreted by a kinetic competition between exportation, folding, and degradation. (C) 2002 Editions scientifiques et medicales Elsevier SAS. All rights reserved.

引用

页码：399 / 404

页数：6

共 25 条

[1]

[Anonymous], 1996, ESCHERICHIA COLI SAL

[2] EXPORT OF THE PERIPLASMIC MALTOSE-BINDING PROTEIN OF ESCHERICHIA-COLI [J].

BASSFORD, PJ .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1990, 22 (03) :401-439

[3] MUTATIONS WHICH ALTER THE FUNCTION OF THE SIGNAL SEQUENCE OF THE MALTOSE BINDING-PROTEIN OF ESCHERICHIA-COLI [J].

BEDOUELLE, H ;

BASSFORD, PJ ;

FOWLER, AV ;

ZABIN, I ;

BECKWITH, J ;

HOFNUNG, M .

NATURE, 1980, 285 (5760) :78-81

[4] IN-VIVO ASSEMBLY OF ACTIVE MALTOSE-BINDING PROTEIN FROM INDEPENDENTLY EXPORTED PROTEIN-FRAGMENTS [J].

BETTON, JM ;

HOFNUNG, M .

EMBO JOURNAL, 1994, 13 (05) :1226-1234

[5] Folding of a mutant maltose-binding protein of Escherichia coli which forms inclusion bodies [J].

Betton, JM ;

Hofnung, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (14) :8046-8052

[6] Probing the structural role of an alpha beta loop of maltose-binding protein by mutagenesis: Heat-shock induction by loop variants of the maltose-binding protein that form periplasmic inclusion bodies [J].

Betton, JM ;

Boscus, D ;

Missiakas, D ;

Raina, S ;

Hofnung, M .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 262 (02) :140-150

[7] Degradation versus aggregation of misfolded maltose-binding protein in the periplasm of Escherichia coli [J].

Betton, JM ;

Sassoon, N ;

Hofnung, M ;

Laurent, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (15) :8897-8902

[8] A newly synthesized, ribosome-bound polypeptide chain adopts conformations dissimilar from early in vitro refolding intermediates [J].

Clark, PL ;

King, J .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (27) :25411-25420

[9] Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli [J].

Danese, PN ;

Silhavy, TJ .

ANNUAL REVIEW OF GENETICS, 1998, 32 :59-94

[10] The structural basis of protein targeting and translocation in bacteria [J].

Driessen, AJM ;

Manting, EH ;

van der Does, C .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (06) :492-498

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