Analysis of the actinobacterial insertion in domain III of the 23S rRNA gene of uncultured variants of the bacterium associated with Whipple's disease using broad-range and 'Tropheryma whippelii'-specific PCR

被引：18

作者：

Hinrikson, HP ^{[1
]}

Dutly, F ^{[1
]}

Altwegg, M ^{[1
]}

机构：

[1] Univ Zurich, Dept Med Microbiol, CH-8028 Zurich, Switzerland

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2000年 / 50卷

关键词：

Whipple's disease; 'Tropheryma whippeli'; actinobacterial insertion in domain III of 23S rDNA; 16S-23S rDNA spacer region; molecular characterization;

D O I：

10.1099/00207713-50-3-1007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Heterogeneity in the 16S-23S rDNA spacer of uncultured 'Tropheryma whippelii' has previously been reported. In this study, the hypervariable insertion in the 23S rDNA domain III characteristic for actinobacteria was analysed. The finding of a unique sequence virtually identical among the subtypes supports the classification of 'T. whippelii' among the actinobacteria and the concept of three subtypes rather than three subspecies, and provides an alternative target for diagnostic assays.

引用

页码：1007 / 1011

页数：5

共 21 条

[1]

Brändle M, 1999, CLIN ENDOCRINOL, V50, P399

[2]

DOBBINS WO, 1987, WHIPPLES DIS

[3] INTERACTION OF RIBOSOMAL-PROTEINS L25 FROM YEAST AND EL23 FROM ESCHERICHIA-COLI WITH YEAST 26S AND MOUSE 28S RIBOSOMAL-RNA [J].

ELBARADI, TTAL ;

DEREGT, VCHF ;

PLANTA, RJ ;

NIERHAUS, KH ;

RAUE, HA .

BIOCHIMIE, 1987, 69 (09) :939-948

[4] THE MOLECULAR PHYLOGENY AND SYSTEMATICS OF THE ACTINOMYCETES [J].

EMBLEY, TM ;

STACKEBRANDT, E .

ANNUAL REVIEW OF MICROBIOLOGY, 1994, 48 :257-289

[5] HOW CLOSE IS CLOSE - 16S RIBOSOMAL-RNA SEQUENCE IDENTITY MAY NOT BE SUFFICIENT TO GUARANTEE SPECIES IDENTITY [J].

FOX, GE ;

WISOTZKEY, JD ;

JURTSHUK, P .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01) :166-170

[6] Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing [J].

Goldenberger, D ;

Kunzli, A ;

Vogt, P ;

Zbinden, R ;

Altwegg, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (11) :2733-2739

[7] LESSONS FROM AN EVOLVING RIBOSOMAL-RNA - 16S AND 23S RIBOSOMAL-RNA STRUCTURES FROM A COMPARATIVE PERSPECTIVE [J].

GUTELL, RR ;

LARSEN, N ;

WOESE, CR .

MICROBIOLOGICAL REVIEWS, 1994, 58 (01) :10-26

[8] A COMPILATION OF LARGE SUBUNIT (23S-LIKE AND 23S-LIKE) RIBOSOMAL-RNA STRUCTURES - 1993 [J].

GUTELL, RR ;

GRAY, MW ;

SCHNARE, MN .

NUCLEIC ACIDS RESEARCH, 1993, 21 (13) :3055-3074

[9] Homogeneity of 16S-23S ribosomal intergenic spacer regions of Tropheryma whippelii in Swiss patients with Whipple's disease [J].

Hinrikson, HP ;

Dutly, F ;

Altwegg, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (01) :152-156

[10] Detection of three different types of 'Tropheryma whippelii' directly from clinical specimens by sequencing, single-strand conformation polymorphism (SSCP) analysis and type-specific PCR of their 16S-23S ribosomal intergenic spacer region [J].

Hinrikson, HP ;

Dutly, F ;

Nair, S ;

Altwegg, M .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :1701-1706

← 1 2 3 →