Heavy metal toxicity and genotoxicity in water and sewage determined by microbiological methods

被引:32
作者
Codina, JC [1 ]
Cazorla, FM [1 ]
Pérez-García, A [1 ]
de Vicente, A [1 ]
机构
[1] Univ Malaga, Fac Ciencias, Dept Microbiol, Malaga 29071, Spain
关键词
toxicity; genotoxicity; heavy metals; sewage; microbial tests;
D O I
10.1002/etc.5620190611
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Acute toxicity and genotoxicity of cadmium, copper, chromium, mercury, nickel, and zinc dissolved in deionized water and in sewage were established by comparing the EC50 and EC20 values obtained by different microbial assays. For acute toxicity determination, The Netherlands Standard NEN6509 test, the spectrophotometric assays of respiratory inhibition using Saccharomyces cerevisiae and Pseudomonas fluorescens, and the Microtox(TM) test were employed. To determine metal genotoxicity, the Salmonella typhimurium and Escherichia coli mutagenicity tests, the SOS-beta-galactosidase genotoxicity test, and the Mutatox(TM) assay were used. The toxicity of the different assayed metals varied from the most toxic, mercury, to the least toxic, nicker and zinc. Two different rankings of toxicity and genotoxicity, very similar to each other, were established. The toxicity ranking was Hg > Cr > Cd similar to Cu similar to Zn > Ni, and the genotoxicity ranking was Hg > Cr > Cu similar to Cd similar to Ni > Zn. The association between the toxicity and genotoxicity of copper and chromium in the dissolved and suspended fractions of sewage was also determined. Copper was mainly associated with the suspended fractions and chromium with the dissolved fractions of sewage.
引用
收藏
页码:1552 / 1558
页数:7
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