Genomic organization and transcriptional regulation of maize ZmRR1 and ZmRR2 encoding cytokinin-inducible response regulators

被引:8
作者
Deji, A
Sakakibara, H [1 ]
Ishida, Y
Yamada, S
Komari, T
Kubo, T
Sugiyama, T
机构
[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Biol Mechanisms & Funct, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[2] Japan Tobacco Inc, Plant Bleeding & Genet Lab, Shizuoka 4280802, Japan
来源
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1492卷 / 01期
关键词
cytokinin; genomic DNA; response regulator; transcriptional regulation; transgenic plant; Zea mays;
D O I
10.1016/S0167-4781(00)00076-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Maize genomic clones encoding cytokinin-inducible response regulators, ZmRR1 and ZmRR2, have been isolated. In comparison with the corresponding cDNAs, ZmRR2 was found to be interrupted in the translated region by an intron whereas ZmRR1 was not. The 5'-flanking regions of the two genes shared conserved regions and putative cis-elements, which had been identified in maize or other plant species. The run-on transcription assay and the analysis of stable maize transformants of ZmRR1 promoter-beta-glucuronidase fusion gene revealed that the accumulation of the transcripts in response to cytokinins is, at least in parts, attributed by transcriptional activation. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:216 / 220
页数:5
相关论文
共 24 条
[1]  
Andersen BR, 1996, PLANTA, V198, P1, DOI 10.1007/BF00197579
[2]  
[Anonymous], 1989, SYNTHETIC OLIGONUCLE
[3]   Meristem, cell division and S phase-dependent activity of wheat histone H4 promoter in transgenic maize plants [J].
Bilgin, M ;
Dedeoglu, D ;
Omirulleh, S ;
Peres, A ;
Engler, G ;
Inzé, D ;
Dudits, D ;
Fehér, A .
PLANT SCIENCE, 1999, 143 (01) :35-44
[4]   Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis [J].
Brandstatter, I ;
Kieber, JJ .
PLANT CELL, 1998, 10 (06) :1009-1019
[5]  
FEINBERG AP, 1984, ANAL BIOCHEM, V137, P266
[6]   ORGAN SPECIFICITY AND LIGHT REGULATION OF NADH-DEPENDENT HYDROXYPYRUVATE REDUCTASE TRANSCRIPT ABUNDANCE [J].
GREENLER, JM ;
BECKER, WM .
PLANT PHYSIOLOGY, 1990, 94 (03) :1484-1487
[7]   THE MYB-HOMOLOGOUS P-GENE CONTROLS PHLOBAPHENE PIGMENTATION IN MAIZE FLORAL ORGANS BY DIRECTLY ACTIVATING A FLAVONOID BIOSYNTHETIC GENE SUBSET [J].
GROTEWOLD, E ;
DRUMMOND, BJ ;
BOWEN, B ;
PETERSON, T .
CELL, 1994, 76 (03) :543-553
[8]   High efficiency transformation of maize (Zea mays L) mediated by Agrobacterium tumefaciens [J].
Ishida, Y ;
Saito, H ;
Ohta, S ;
Hiei, Y ;
Komari, T ;
Kumashiro, T .
NATURE BIOTECHNOLOGY, 1996, 14 (06) :745-750
[9]  
JACQMARD A, 1994, CYTOKININS CHEM ACTI, P197
[10]   Interaction of DNA-binding proteins with the 5′-flanking region of a cytokinin-responsive cucumber hydroxypyruvate reductase gene [J].
Jin, G ;
Davey, MC ;
Ertl, JR ;
Chen, R ;
Yu, ZT ;
Daniel, SG ;
Becker, WM ;
Chen, CM .
PLANT MOLECULAR BIOLOGY, 1998, 38 (05) :713-723