Biosynthesis and Biological Screening of a Genetically Encoded Library Based on the Cyclotide MCoTI-I

被引：77

作者：

Austin, Jeffrey ^{[2
]}

Wang, Wan ^{[1
]}

Puttamadappa, Swamy ^{[3
]}

Shekhtman, Alexander ^{[3
]}

Camarero, Julio A. ^{[1
,2
]}

机构：

[1] Univ So Calif, Dept Pharmacol & Pharmaceut Sci, Sch Pharm, Los Angeles, CA 90033 USA

[2] Lawrence Livermore Natl Lab, Livermore, CA 94550 USA

[3] SUNY Albany, Dept Chem, Albany, NY 12222 USA

来源：

CHEMBIOCHEM | 2009年 / 10卷 / 16期

关键词：

cyclotides; expressed protein ligation; genetically encoded libraries; inhibitors; protein splicing; CYCLIC-CYSTINE-KNOT; COMBINATORIAL PEPTIDE TEMPLATE; POLYPEPTIDE KALATA B1; TRYPSIN-INHIBITOR; DRUG DESIGN; MOMORDICA-COCHINCHINENSIS; MACROCYCLIC PEPTIDES; CHEMICAL-SYNTHESIS; PROTEIN; CYCLIZATION;

D O I：

10.1002/cbic.200900534

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) Cell-ing point: This study shows that MCoTI-cyclotides can provide an ideal scaffold for the biosynthesis of large combinatorial libraries inside living E. coli cells. Coupled to an appropriate in vivo reporter system, this library may rapidly be screened, for example, by fluorescence-activated cell sorting. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：2663 / 2670

页数：8

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