Sequential G-banding FISH on human sperm chromosomes

被引：5

作者：

Colls, P

Templado, C

MartinezPasarell, O

Darroudi, F

Natarajan, AT

机构：

[1] UNIV AUTONOMA BARCELONA, FAC MED, DEPT BIOL CELULAR & FISIOL, BELLATERRA 08193, BARCELONA, SPAIN

[2] INTERUNIV RES INST RADIOPATHOL & RADIAT PROTECT, JA COHEN INST, LEIDEN, NETHERLANDS

来源：

CHROMOSOME RESEARCH | 1997年 / 5卷 / 07期

关键词：

double-colour FISH; G-banding; human sperm chromosomes;

D O I：

10.1023/A:1018464929628

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method that allows the performance of double-colour chromosome painting (FISH) on previously G-banded human sperm metaphases has been developed. Sperm chromosomes were obtained by using the fusion technique between zona-free hamster oocytes and human spermatozoa. Single-and double-colour chromosome painting was performed using DNA libraries specific for chromosomes X, Y and 21 on either unstained or G-banded preparations. The hybridization efficiency was very high (98%). The sequential staining technique is very useful for analyses of structural (stable) and numerical chromosome aberrations in human sperm and thus can increase the efficiency of the human sperm-hamster oocytes fusion system to assess the risk to human germ cells as a result of endogenous and exogenous factors.

引用

页码：457 / 461

页数：5

共 26 条

[1] A FLUORESCENCE INSITU HYBRIDIZATION TECHNIQUE FOR RETROSPECTIVE CYTOGENETIC ANALYSIS [J].