Effects of ozone on macrophage adhesion in vitro and epithelial and inflammatory responses in vivo: The role of cytokines

被引:26
作者
Pearson, AC
Bhalla, DK
机构
[1] WAYNE STATE UNIV, DETROIT, MI 48202 USA
[2] UNIV CALIF IRVINE, DEPT COMMUNITY & ENVIRONM MED, IRVINE, CA 92717 USA
来源
JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH | 1997年 / 50卷 / 02期
关键词
D O I
10.1080/009841097160546
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Inhalation exposure to ozone (O-3) is known to induce epithelial and inflammatory changes in the lungs, characterized by neutrophilia and changes in epithelial permeability. Several cell types and their soluble mediators, including interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-alpha), are involved in the evolution of these responses. In this study, we have compared the effects of the combination of anti-IL-1 alpha and anti-TNF-alpha on in vitro and in vivo responses to inhaled O-3. Male, Sprague-Dawley rats were exposed, nose-only, to 0.8 ppm O-3 for 3 h and the in vitro and in vivo parameters were measured 8-12 h following exposure. The in vitro studies revealed that the adherence of inflammatory cells, primarily macrophages, harvested from the lungs of O-3-exposed rats to cultured lung epithelial cells (ARL-14) was significantly greater than the adherence of macrophages from air-exposed controls. Furthermore, this adherence was significantly reduced in antibody-treated cells as compared to cells treated with preimmune rabbit serum. In vivo, elevations were found in the percentage of neutrophils in bronchoalveolar lavage fluid (BALF), transport of Tc-99m-diethylenetriaminepentaacetate (DTPA) across the tracheal epithelium, and concentrations of total protein and albumin in BALF following O-3 exposure. However, these effects were not significantly altered by treatment with the anti-IL-1 alpha/anti-TNF-alpha combination. Therefore it was concluded that O-3 affects the early stages of the inflammatory response, particularly with respect to macrophage activation and adherence to epithelial cells, and that this early response may be mediated by IL-1 alpha and/or TNF-alpha. The results also suggest that the in vivo effects of O-3 are controlled by complex mechanisms involving factors other than IL-1 alpha and TNF-alpha, even though these cytokines are capable of modifying macrophage function as revealed by the in vitro adherence studies.
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收藏
页码:143 / 157
页数:15
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