S100 protein-immunoreactive primary sensory neurons in the trigeminal and dorsal root ganglia of the rat

被引:52
作者
Ichikawa, H [1 ]
Jacobowitz, DM [1 ]
Sugimoto, T [1 ]
机构
[1] NIMH,CLIN SCI LAB,BETHESDA,MD 20892
关键词
S100; parvalbumin; calbindin D-28k; calretinin; trigeminal ganglion; dorsal root ganglion;
D O I
10.1016/S0006-8993(96)01364-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The cell body size (cross-sectional area) of S100-immunoreactive (-ir) primary neurons was measured in the trigeminal (TG) and lumbar dorsal root ganglia (DRG). About a half of neurons exhibited S100-immunoreactivity (-ir) in the DRG (44.0%) and TG (59.0%). DRG neurons with cell bodies >1200 mu m(2) mostly exhibited S100-ir (96.5%), whereas S100-ir DRG neurons <600 mu m(2) were rare (8.0%). 36.6% of DRG neurons in the cell size range 600-1200 mu m(2) showed the ir. TG neurons >800 mu m(2) mostly exhibited S100-ir (93.1%), whereas those <400 mu m(2) were devoid of it (positive cells 10.5%). 58.3% of TG cells in the range 400-800 mu m(2) contained S100-ir. Double-immunofluorescence method revealed the co-expression of S100 and other calcium-binding proteins. Parvalbumin-ir neurons mostly exhibited S100-ir in the DRG (97.4%) and TG (97.0%). The co-expression of S100 and calbindin D-28k was very rare in the DRG, because the DRG contained few calbindin D-28k-ir neurons. Unlike in the DRG, numerous neurons co-expressed S100- and calbindin D-28k-ir in the TG. Most calbindin D-28k-ir TG neurons were also immunoreactive for S100 (90.7%). Sub-populations of calretinin (CR)-ir neurons co-expressed S100-ir in both the DRG (68%) and TG (50.0%). Virtually all CR-ir neurons > 1400 mu m(2) co-expressed S100-ir in the DRG (100%) and TG (95.9%). CR-ir neurons <800 mu m(2) were rarely exhibited S100-ir (DRG 18.0%, TG 21.9%). 71.3 and 60.5% of CR-ir neurons in the range 800-1400 mu m(2) co-expressed S100-ir in the DRG and TG, respectively. The present study indicates that S100 is closely correlated to the primary neuronal cell size in the DRG and TG.
引用
收藏
页码:253 / 257
页数:5
相关论文
共 21 条
[1]   ANALYSIS OF PARVALBUMIN AND CALBINDIN D28K-IMMUNOREACTIVE NEURONS IN DORSAL-ROOT GANGLIA OF RAT IN RELATION TO THEIR CYTOCHROME-OXIDASE AND CARBONIC-ANHYDRASE CONTENT [J].
CARR, PA ;
YAMAMOTO, T ;
KARMY, G ;
BAIMBRIDGE, KG ;
NAGY, JI .
NEUROSCIENCE, 1989, 33 (02) :363-371
[2]   CALBINDIN-D-28K AND PARVALBUMIN IN THE RAT NERVOUS-SYSTEM [J].
CELIO, MR .
NEUROSCIENCE, 1990, 35 (02) :375-475
[3]   PERIPHERAL PROJECTIONS OF CALRETININ-IMMUNOREACTIVE PRIMARY SENSORY NEURONS IN CHICK HINDLIMBS [J].
DUC, C ;
BARAKATWALTER, I ;
DROZ, B .
BRAIN RESEARCH, 1993, 622 (1-2) :321-324
[4]   CALBINDIN-D-28K-IMMUNOREACTIVE AND SUBSTANCE-P-IMMUNOREACTIVE PRIMARY SENSORY NEURONS - PERIPHERAL PROJECTIONS IN CHICK HINDLIMBS [J].
DUC, C ;
BARAKATWALTER, I ;
DROZ, B .
JOURNAL OF COMPARATIVE NEUROLOGY, 1993, 334 (01) :151-158
[5]   DIFFERENTIAL DISTRIBUTION OF CALBINDIN-D-28K AND PARVALBUMIN IN SOMATIC AND VISCERAL SENSORY NEURONS [J].
HONDA, CN .
NEUROSCIENCE, 1995, 68 (03) :883-892
[6]   CALRETININ-IMMUNOREACTIVITY IN THE OROFACIAL AND PHARYNGEAL REGIONS OF THE RAT [J].
ICHIKAWA, H ;
JACOBOWITZ, DM ;
SUGIMOTO, T .
NEUROSCIENCE LETTERS, 1992, 146 (02) :155-158
[7]   CALRETININ-IMMUNOREACTIVE NEURONS IN THE TRIGEMINAL AND DORSAL-ROOT GANGLIA OF THE RAT [J].
ICHIKAWA, H ;
JACOBOWITZ, DM ;
SUGIMOTO, T .
BRAIN RESEARCH, 1993, 617 (01) :96-102
[8]   NEURAL PARVALBUMIN AND CALRETININ IN THE TOOTH-PULP [J].
ICHIKAWA, H ;
DEGUCHI, T ;
MITANI, S ;
NAKAGO, T ;
JACOBOWITZ, DM ;
YAMAAI, T ;
SUGIMOTO, T .
BRAIN RESEARCH, 1994, 647 (01) :124-130
[9]   Calbindin-D28k-immunoreactivity in the trigeminal ganglion neurons and molar tooth pulp of the rat [J].
Ichikawa, H ;
Deguchi, T ;
Fujiyoshi, Y ;
Nakago, T ;
Jacobowitz, DM ;
Sugimoto, T .
BRAIN RESEARCH, 1996, 715 (1-2) :71-78
[10]   PARVALBUMIN, CALRETININ AND CARBONIC-ANHYDRASE IN THE TRIGEMINAL AND SPINAL PRIMARY NEURONS OF THE RAT [J].
ICHIKAWA, H ;
DEGUCHI, T ;
NAKAGO, T ;
JACOBOWITZ, DM ;
SUGIMOTO, T .
BRAIN RESEARCH, 1994, 655 (1-2) :241-245