Multi-stage chemical carcinogenesis in mouse skin: Fundamentals and applications

被引:405
作者
Abel, Erika L. [1 ]
Angel, Joe M. [1 ]
Kiguchi, Kaoru [1 ]
DiGiovanni, John [1 ]
机构
[1] Univ Texas MD Anderson Canc Ctr, Dept Carcinogenesis, Div Sci Pk Res, Smithville, TX USA
关键词
SQUAMOUS-CELL CARCINOMA; ENHANCES MALIGNANT PROGRESSION; KERATINOCYTE STEM-CELLS; HARVEY-RAS GENE; TUMOR PROMOTION; SENCAR MICE; EPIDERMAL-KERATINOCYTES; 2-STAGE CARCINOGENESIS; C57BL/6; MICE; CYCLOOXYGENASE-2; OVEREXPRESSION;
D O I
10.1038/nprot.2009.120
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
For more than 60 years, the chemical induction of tumors in mouse skin has been used to study mechanisms of epithelial carcinogenesis and evaluate modifying factors. In the traditional two-stage skin carcinogenesis model, the initiation phase is accomplished by the application of a sub-carcinogenic dose of a carcinogen. Subsequently, tumor development is elicited by repeated treatment with a tumor-promoting agent. The initiation protocol can be completed within 1-3 h depending on the number of mice used; whereas the promotion phase requires twice weekly treatments (1-2 h) and once weekly tumor palpation (1-2 h) for the duration of the study. Using the protocol described here, a highly reproducible papilloma burden is expected within 10-20 weeks with progression of a portion of the tumors to squamous cell carcinomas within 20-50 weeks. In contrast to complete skin carcinogenesis, the two-stage model allows for greater yield of premalignant lesions, as well as separation of the initiation and promotion phases.
引用
收藏
页码:1350 / 1362
页数:13
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