Super-Resolution Imaging with Small Organic Fluorophores

被引：313

作者：

Heilemann, Mike ^{[1
]}

van de Linde, Sebastian ^{[1
]}

Mukherjee, Anindita ^{[1
]}

Sauer, Markits ^{[1
]}

机构：

[1] Univ Bielefeld, D-33615 Bielefeld, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2009年 / 48卷 / 37期

关键词：

diffraction limit; dyes/pigments; live-cell imaging; photoswitching; super-resolution imaging; GROUND-STATE-DEPLETION; FLUORESCENCE NANOSCOPY; AROMATIC THIOLS; MICROSCOPY; MOLECULES; DYES; GLUTATHIONE; OXIDATION; PROTEINS; BREAKING;

D O I：

10.1002/anie.200902073

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

What's your color? A universal and facile method for reversible photoswitching of commercially available Alexa Fluor and ATTO dyes spanning the entire visible range yields an optical resolution of approximately 20 nm. The intriguing simplicity of the method facilitates applications and opens avenues for multicolor super-resolution imaging in fixed and living cells. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：6903 / 6908

页数：6

共 41 条

[1]

Abbe E., 1873, Arch. Mikrosk. Anat., V9, P413, DOI DOI 10.1007/BF02956173

[2]

[Anonymous], 2008, ANGEW CHE

[3] Multicolor super-resolution imaging with photo-switchable fluorescent probes [J].