Structure determination of tubular crystals of membrane proteins. II. Averaging of tubular crystals of different helical classes

被引：15

作者：

Yonekura, K ^{[1
]}

Toyoshima, C ^{[1
]}

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1130032, Japan

来源：

ULTRAMICROSCOPY | 2000年 / 84卷 / 1-2期

关键词：

cryo-electron microscopy; tubular crystal; real-space averaging; Ca2+-ATPase;

D O I：

10.1016/S0304-3991(00)00009-7

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A set of programs has been developed for averaging the data from tubular crystals belonging to different helical classes. This was done either by (i) cutting out molecules constituting a unit cell from density maps, and aligning and averaging them in real space; (ii) transforming the densities in a unit cell to layer-line data according to a (possibly artificial) helical symmetry, aligning and averaging them in reciprocal space. These methods were applied to tubular crystals of Ca2+-ATPase. Either method worked well and substantially improved the data quality. Transforming the reconstructed images to the layer-line data has many advantages and is essential for fully exploiting the power of averaging. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：15 / 28

页数：14

共 25 条

[1] Three-dimensional structure of bovine cytochrome bc(1) complex by electron cryomicroscopy and helical image reconstruction [J].