Total glucosides of peony improve ovalbumin-induced allergic asthma by inhibiting mast cell degranulation

被引：26

作者：

Shou, Qiyang ^{[1
,2
]}

Lang, Jiali ^{[1
,2
]}

Jin, Lu ^{[1
,2
]}

Fang, Mingsun ^{[3
]}

Cao, Beibei ^{[1
]}

Cai, Yueqin ^{[3
]}

Ni, Zhunan ^{[4
]}

Qiu, Fengmei ^{[2
]}

Li, Changyu ^{[3
]}

Cao, Gang ^{[2
]}

Fu, Huiying ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Affiliated Secondary Hosp, Clin Med Coll 2, Hangzhou, Zhejiang, Peoples R China

[2] Zhejiang Chinese Med Univ, Sch Pharm, Hangzhou, Zhejiang, Peoples R China

[3] Zhejiang Chinese Med Univ, Acad Chinese Med, Hangzhou, Zhejiang, Peoples R China

[4] Zhejiang Prov Ctr Dis Control & Prevent, Hangzhou, Zhejiang, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2019年 / 244卷

基金：

中国国家自然科学基金;

关键词：

Total glucosides of peony; Asthma; Mast cell; Immunity;

D O I：

10.1016/j.jep.2019.112136

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Ethnopharmacological relevance: Paeonia lactiflora Pall. (peony) is a medicinal plant used in the Xiaoqinglong decoction, a commonly prescribed traditional Chinese medicine for asthma. The main active ingredients of peony roots-described as the total glucosides of peony (TGP)-have anti-inflammatory, immunomodulatory, and protective effects on endothelial cells, and they are known to improve rheumatoid arthritis. This study explored the underlying mechanism of TGP activity in the treatment of allergic asthma. Materials and methods: Allergic asthma was induced in BALB/c mice by administering injections of ovalbumin (OVA) mixed with aluminum hydroxide gel and inhaling nebulized OVA. The OVA-sensitized mice were treated with TGP by oral gavage, and the potentially anti-asthmatic treatment effect was studied by testing airway hyperresponsiveness, classifying and counting of leukocytes, performing cytokine assays, and analyzing the lung histopathology. The beta-hexosaminidase activity was assayed as a biomarker to evaluate the effect of TGP on mast cell degranulation. The mechanism of TGP was explored by monitoring the Ca2+ influx level in mast cells (RBL-2H3) using a Ca2+ fluorescent probe technique. Results: In mice with OVA-induced allergic asthma, TGP reduced airway hyperresponsiveness and improved lung tissue pathology, which included a decrease in inflammatory cell infiltration and collagen deposition. TGP also significantly lowered BALF leukocyte, eosinophil, and neutrophil counts, along with chemokines and cytokines, such as eotaxin, TNF-alpha, IL-4, and MIP-1 alpha, in serum and lungs of OVA-challenged mice. These effects were further confirmed with the decrease of beta-hexosaminidase release and the inhibition of Ca2+ influx in mast cell degranulation. Conclusions: Our findings suggest that TGP improved OVA-induced allergic asthma in mice mainly by suppressing Ca2+ influx-dependent mast cell degranulation.

引用

页数：8

共 24 条

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