Condition-dependent presence of β-lipotropin-like peptide in human keratinocytes

被引：11

作者：

Wintzen, M

Zanello, SB

Holick, MF

Wiegant, VM

Burbach, JPH

Vermeer, BJ

机构：

[1] Leiden Univ, Med Ctr, Dept Dermatol, NL-2333 ZA Leiden, Netherlands

[2] Boston Univ, Sch Med, Dept Med, Boston, MA 02118 USA

[3] Univ Utrecht, Dept Med Pharmacol, Rudolf Magnus Inst Neurosci, Utrecht, Netherlands

来源：

PEPTIDES | 2000年 / 21卷 / 05期

关键词：

beta-endorphin; beta-lipotropin; POMC; radioimmunoassay; HPLC; human keratinocyte; culture; ultraviolet-radiation;

D O I：

10.1016/S0196-9781(00)00193-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study aimed to characterize the beta-endorphin-immunoreactive material (beta E-IR) detectable in normal human keratinocytes (NHK). The effects of different culturing conditions and UV-irradiation on production of beta E-IR by NHK were assessed by radioimmunoassay and HPLC. All culture systems contained low levels of beta E-IR that was increased in conditioned media after UV-irradiation under certain conditions. NHK grown in nutrient-poor medium contained highest levels of beta E-IR that exhibited beta-lipotropin-like properties after HPLC analysis. The other culturing conditions displayed no authentic beta E-related peptides. Our results indicate that under certain culturing conditions NHK can produce POMC peptides like beta-lipotropin, which can be induced by UV-radiation. (C) 2000 Elsevier Science Inc. All rights reserved.

引用

页码：691 / 697

页数：7

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