In vivo visualization of F-actin structures during the development of the moss Physcomitrella patens

被引:30
作者
Finka, Andrija
Schaefer, Didier G.
Saidi, Younousse
Goloubinoff, Pierre
Zryd, Jean-Pierre
机构
[1] Ecole Polytech Fed Lausanne, SW IBL, CH-1015 Lausanne, Switzerland
[2] INRA, Genet & Ameliorat Plantes Stn, F-78026 Versailles, France
[3] Univ Lausanne, Dept Plant Mol Biol, CH-1015 Lausanne, Switzerland
关键词
actin microfilament; ARP3; cell polarity; cytoskeleton; green fluorescent protein (GFP)-talin; heat-shock promoter; moss; Physcomitrella patens;
D O I
10.1111/j.1469-8137.2007.01989.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The 'in planta' visualization of F-actin in all cells and in all developmental stages of a plant is a challenging problem. By using the soybean heat inducible Gmhsp17.3B promoter instead of a constitutive promoter, we have been able to label all cells in various developmental stages of the moss Physcomitrella patens, through a precise temperature tuning of the expression of green fluorescent protein (GFP)-talin. A short moderate heat treatment was sufficient to induce proper labeling of the actin cytoskeleton and to allow the visualization of time-dependent organization of F-actin structures without impairment of cell viability. In growing moss cells, dense converging arrays of F-actin structures were present at the growing tips of protonema cell, and at the localization of branching. Protonema and leaf cells contained a network of thick actin cables; during de-differentiation of leaf cells into new protonema filaments, the thick bundled actin network disappeared, and a new highly polarized F-actin network formed. The controlled expression of GFP-talin through an inducible promoter improves significantly the 'in planta' imaging of actin.
引用
收藏
页码:63 / 76
页数:14
相关论文
共 42 条
[1]  
Alessa L, 1999, DEVELOPMENT, V126, P201
[2]   ISOLATION AND PRELIMINARY CHARACTERIZATION OF AUXOTROPHIC AND ANALOG RESISTANT MUTANTS OF MOSS, PHYSCOMITRELLA-PATENS [J].
ASHTON, NW ;
COVE, DJ .
MOLECULAR & GENERAL GENETICS, 1977, 154 (01) :87-95
[3]  
BASKIN TI, 1994, PLANT CELL PHYSIOL, V35, P935
[4]  
BASKIN TI, 1995, PLANTA, V197, P514, DOI 10.1007/BF00196673
[5]  
Cove DJ, 1992, DEV MOL GENETIC APPR, P179
[6]   ADF proteins are involved in the control of flowering and regulate F-actin organization, cell expansion, and organ growth in Arabidopsis [J].
Dong, CH ;
Xia, GX ;
Hong, Y ;
Ramachandran, S ;
Kost, B ;
Chua, NH .
PLANT CELL, 2001, 13 (06) :1333-1346
[7]  
DOONAN JH, 1988, J CELL SCI, V89, P533
[8]   DISTORTED2 encodes an ARPC2 subunit of the putative Arabidopsis ARP2/3 complex [J].
El-Assall, SE ;
Le, J ;
Basu, D ;
Mallery, EL ;
Szymanski, DB .
PLANT JOURNAL, 2004, 38 (03) :526-538
[9]   Rop GTPase-dependent dynamics of tip-localized F-actin controls tip growth in pollen tubes [J].
Fu, Y ;
Wu, G ;
Yang, ZB .
JOURNAL OF CELL BIOLOGY, 2001, 152 (05) :1019-1032
[10]   The cytoskeleton in plant and fungal cell tip growth [J].
Geitmann, A ;
Emons, AMC .
JOURNAL OF MICROSCOPY, 2000, 198 :218-245