Over expression of endoglin in human prostate cancer suppresses cell detachment, migration and invasion

被引:89
作者
Liu, YQ
Jovanovic, B
Pins, M
Lee, C
Bergan, RC
机构
[1] Northwestern Univ, Sch Med, Dept Med, Div Hematol Oncol, Chicago, IL 60611 USA
[2] Northwestern Univ, Robert H Lurie Canc Ctr, Chicago, IL 60611 USA
[3] Northwestern Univ, Sch Med, Dept Prevent Med, Chicago, IL 60611 USA
[4] Northwestern Univ, Sch Med, Dept Pathol, Chicago, IL 60611 USA
[5] Northwestern Univ, Sch Med, Dept Urol, Chicago, IL 60611 USA
关键词
adhesion; motility; transforming growth factor beta; carcinogenesis;
D O I
10.1038/sj.onc.1206117
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The regulation of cell adhesion and motility in human prostate is not well understood. We have previously shown that the endoglin gene is differently expressed during changes in prostate cell adhesion. Endoglin is a transmembrane transforming growth factor beta binding protein typically expressed by endothelial cells. In this report we demonstrate that endoglin over expression increases prostate cell attachment, while decreasing migration and invasion. Engineered decreases in endoglin expression have opposite effects. While endoglin exerted only relatively small effects upon cell adhesion, large effects upon cell migration and invasion were observed. Endoglin was shown to localize to focal adhesion plaques, consistent with its role in regulating cell adhesion and motility. Loss of endoglin expression in cancer, as compared to normal prostate, was seen in human prostate cell lines. Suppression of endoglin expression in a panel of normal human prostate cell lines led to cell detachment. Endoglin is identified as a regulator of cell adhesion, motility and invasion in human prostate. Loss of endoglin expression appears to be associated with prostate cancer progression, at least in vitro.
引用
收藏
页码:8272 / 8281
页数:10
相关论文
共 51 条
[1]   Thrombospondin-1 and transforming growth factor-beta1 promote breast tumor cell invasion through upregulation of the plasminogen/plasmin system [J].
Albo, D ;
Berger, DH ;
Wang, TN ;
Hu, XL ;
Rothman, V ;
Tuszynski, GP .
SURGERY, 1997, 122 (02) :493-499
[2]   Expression and structural features of endoglin (CD105), a transforming growth factor beta 1 and beta 3 binding protein, in human melanoma [J].
Altomonte, M ;
Montagner, R ;
Fonsatti, E ;
Colizzi, F ;
Cattarossi, I ;
Brasoveanu, LI ;
Nicotra, MR ;
Cattelan, A ;
Natali, PG ;
Maio, M .
BRITISH JOURNAL OF CANCER, 1996, 74 (10) :1586-1591
[3]   Endoglin is an accessory protein that interacts with the signaling receptor complex of multiple members of the transforming growth factor-β superfamily [J].
Barbara, NP ;
Wrana, JL ;
Letarte, M .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (02) :584-594
[4]   IDENTIFICATION AND EXPRESSION OF 2 FORMS OF THE HUMAN TRANSFORMING GROWTH FACTOR-BETA-BINDING PROTEIN ENDOGLIN WITH DISTINCT CYTOPLASMIC REGIONS [J].
BELLON, T ;
CORBI, A ;
LASTRES, P ;
CALES, C ;
CEBRIAN, M ;
VERA, S ;
CHEIFETZ, S ;
MASSAGUE, J ;
LETARTE, M ;
BERNABEU, C .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (09) :2340-2345
[5]   Genistein-stimulated adherence of prostate cancer cells is associated with the binding of focal adhesion kinase to beta-1-integrin [J].
Bergan, R ;
Kyle, E ;
Nguyen, P ;
Trepel, J ;
Ingui, C ;
Neckers, L .
CLINICAL & EXPERIMENTAL METASTASIS, 1996, 14 (04) :389-398
[6]   ELECTROPORATION ENHANCES C-MYC ANTISENSE OLIGODEOXYNUCLEOTIDE EFFICACY [J].
BERGAN, R ;
CONNELL, Y ;
FAHMY, B ;
NECKERS, L .
NUCLEIC ACIDS RESEARCH, 1993, 21 (15) :3567-3573
[7]   APTAMERIC INHIBITION OF P210(BCR-ABL) TYROSINE KINASE AUTOPHOSPHORYLATION BY OLIGODEOXYNUCLEOTIDES OF DEFINED SEQUENCE AND BACKBONE STRUCTURE [J].
BERGAN, R ;
CONNELL, Y ;
FAHMY, B ;
KYLE, E ;
NECKERS, L .
NUCLEIC ACIDS RESEARCH, 1994, 22 (11) :2150-2154
[8]   Electroporation of synthetic oligodeoxynucleotides: A novel technique for ex vivo bone marrow purging [J].
Bergan, R ;
Hakim, F ;
Schwartz, GN ;
Kyle, E ;
Cepada, R ;
Szabo, JM ;
Fowler, D ;
Gress, R ;
Neckers, L .
BLOOD, 1996, 88 (02) :731-741
[9]  
Bright RK, 1997, CANCER RES, V57, P995
[10]   TGF-β1 modulated the expression of α5β1 integrin and integrin-mediated signaling in human hepatocarcinoma cells [J].
Cai, T ;
Lei, QY ;
Wang, LY ;
Zha, XL .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 274 (02) :519-525