Purification of limonoid glucosyltransferase from navel orange albedo tissues

被引：29

作者：

Hasegawa, S

Suhayda, CG

Hsu, WJ

Robertson, GH

机构：

[1] Western Regional Research Center, Agricultural Research Service, USDA, Albany, CA 94710

来源：

PHYTOCHEMISTRY | 1997年 / 46卷 / 01期

关键词：

Citrus sinensis; Rutaceae; limonoids; limonoid glucosides; UDP-D-glucose:limonoid glucosyltransferase;

D O I：

10.1016/S0031-9422(97)00216-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

UDP-D-glucose:limonoid glucosyltransferase was purified from albedo tissues of navel orange (Citrus sinensis) cultivars, Frost and Newhall, by a combination of (NH4)(2)SO4 fractionation, UDP-glucuronic acid affinity chromatography and DEAE ion exchange HPLC. This procedure resulted in a 452-fold increase in enzyme purification. This enzyme catalysed the glucosylation of both nomilin and limonin. SDS-PAGE showed a M-r of 56-58 k for the enzyme. The enzyme displayed a peak of activity between pH 6.5 and 9.0 with an optimum at 8.0. Mn2+ stimulated enzyme activity by 66% over basal activity observed with EDTA. Activity was lost when the purified enzyme was frozen and stored in Tris-HCl buffer at pH 8.0. Published by Elsevier Science Ltd.

引用

页码：33 / 37

页数：5

共 23 条

[1]

BARPELED M, 1991, J BIOL CHEM, V266, P20953

[2] PARTIAL-PURIFICATION, PROPERTIES, AND KINETIC-STUDIES OF UDP-GLUCOSE-P-HYDROXYBENZOATE GLUCOSYLTRANSFERASE FROM CELL-CULTURES OF LITHOSPERMUM-ERYTHRORHIZON [J].