Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP)

被引：632

作者：

Kim, NW

Wu, F

机构：

[1] Geron Corporation, Menlo Park

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 13期

关键词：

D O I：

10.1093/nar/25.13.2595

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomerase activity.

引用

页码：2595 / 2597

页数：3

共 7 条

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