Manure enhances plasmid mobilization and survival of Pseudomonas putida introduced into field soil

We report a field study on plasmid mobilization in an agricultural soil, The influence of Dig manure on the mobilization of the IncQ plasmid pIE723 by indigenous plasmids or by the IncP alpha plasmid pGP527 into the recipient Pseudomonas putida UWC1 (Rif(r) NaIr) was studied in field soil, Sh plots were prepared in duplicate, three of which were treated with manure prior to inoculation of the donor and recipient strains. As a donor strain, either Escherichia coli J53(pIE723) or E. coli 600(pIE723, pGP527) was used, Potative transconjugants obtained on a selective medium were confirmed by DNA hybridization and PCR. Plasmid mobilization by indigenous mobilizing plasmids was observed on two occasions in manured soil. Manuring of soil significantly enhanced the frequency of pIE723 mobilization by pGP527, since mobilization frequencies into P. putida UWC1 were at least 10-fold higher in manured soil than in nonmanured soil, Enhanced numbers of P, putida UWC1 transconjugant and recipient colonies could be observed in manured soil throughout the 79-day field test, Transfer of pIE723 or pG527 into indigenous soil or rhizosphere bacteria could not be detected when indigenous bacteria isolated by selective cultivation were screened for the presence of these plasmids by DNA hybridization, Furthermore, the presence of IncN-, IncP-, or IncQ-specific sequences was confirmed in total community DNA extracted directly from the manured or nonmanured soil by PCR, IncW plasmids were detectable only in manured soil, indicating entry of these plasmids into soil via manure.