Detection of the oxidative products of 3-hydroxykynurenine using high-performance liquid chromatography electrochemical detection ultraviolet absorption detection electron spin resonance spectrometry and high-performance liquid chromatography electrochemical detection ultraviolet absorption detection mass spectrometry

被引：34

作者：

Iwahashi, H

Ishii, T

机构：

[1] Department of Chemistry, Wakayama Med. College, 651 Hironishi, Wakayama

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 773卷 / 1-2期

关键词：

electrochemical detection; detection; LC; 3-hydroxykynurenine; xanthommatin; hydroxanthommatin;

D O I：

10.1016/S0021-9673(97)00271-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Several oxidative products of 3-hydroxy-DL-kynurenine (3-DL-HKY) were detected using high-performance liquid chromatography-electrochemical detection-ultraviolet absorption detection-electron spin resonance spectrometry (HPLC-ED-UV-ESR) and high-performance liquid chromatography-electrochemical detection-ultraviolet absorption detection-mass spectrometry (HPLC-ED-UV-MS). In the HPLC-ED-UV-ESR and the HPLC-ED-UV-MS systems, the ED electrode is placed between an HPLC injector and an HPLC column, and is used as a reactor. 3-DL-HKY was oxidized by the ED electrode with various applied potentials (0-1.0 V). The oxidative products formed were separated by the HPLC column and then detected by the ultraviolet absorption detector, the electron spin resonance spectrometer and the mass spectrometer, respectively. Thus, the HPLC-ED-UV-ESR and the HPLC-ED-UV-MS systems allow us to separate and identify some of the relatively unstable products, including free radical species that form in the redox reactions of 3-DL-HKY. (C) 1997 Elsevier Science B.V.

引用

页码：23 / 31

页数：9

共 23 条

[1] CANCER OF THE URINARY BLADDER INDUCED IN MICE WITH METABOLITES OF AROMATIC AMINES AND TRYPTOPHAN [J].