A pragmatic and readily implemented quality control strategy for HPLC-MS and GC-MS-based metabonomic analysis

被引：480

作者：

Sangster, Timothy

Major, Hilary

Plumb, Robert

Wilson, Amy J.

Wilson, Ian D.

机构：

[1] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

[2] Waters Corp, Manchester M22 5PP, Lancs, England

[3] Waters Corp, Milford, MA 01757 USA

来源：

ANALYST | 2006年 / 131卷 / 10期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1039/b604498k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Metabonomic/ metabolomic studies can involve the analysis of large numbers of samples for the detection of biomarkers and confidence in the analytical data, generated by methods such as GC and HPLC-MS, requires active measures on the part of the analyst. However, quality control for complex multi-component samples such as biofluids, where many of the components of interest in the sample are unknown prior to analysis, poses significant problems. Here the repeat analysis of a pooled sample throughout the run, thereby enabling the analysis to be monitored and controlled using targeted inspection of the data and pattern recognition, is advocated as a pragmatic solution to this problem.

引用

页码：1075 / 1078

页数：4

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