Conjugative transfer facilitates stable maintenance of IncP-1 plasmid pKJK5 in Escherichia coli cells colonizing the gastrointestinal tract of the germfree rat

被引:19
作者
Bahl, Martin Iain
Hansen, Lars Hestbjerg
Licht, Tine Rask
Sorensen, Soren J.
机构
[1] Univ Copenhagen, Dept Microbiol, DK-1307 Copenhagen, Denmark
[2] Danish Hydraul Food & Vet Res, Dept Microbiol Food Safety, DK-2860 Soborg, Denmark
关键词
D O I
10.1128/AEM.01971-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要
Quantitative determination of IncP-1 plasmid loss from Escherichia coli cells colonizing the gastrointestinal tracts of germfree rats was achieved by flow cytometry. Results show that the plasmid's ability to conjugate counteracts plasmid loss and is thus an important mechanism for the stable maintenance of 1RcP-1 plasmids within the gastrointestinal environment.
引用
收藏
页码:341 / 343
页数:3
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