High yield DNA extraction from the snake cast-off skin or bird feathers using collagenase

被引：15

作者：

Eguchi, T

Eguchi, Y ^{[1
]}

机构：

[1] Univ Ryukyus, Fac Med, Res Lab Ctr, Okinawa 90301, Japan

[2] Univ Ryukyus, Fac Med, Dept Biochem, Okinawa 90301, Japan

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 13期

关键词：

cast-off skin; collagenase; DNA extraction; feather;

D O I：

10.1023/A:1005658029607

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple method to extract DNA in high yield from the snake cast-off skin or bird feathers was developed. The molecular weight of the extracted DNA was higher by this than the conventional method and the yield of DNA was increased by more than one hundred fold. The DNA extracted by this method could be used for PCR and other analyses. This method could be applied to various samples, for instance, extracting DNA from bird feather in general.

引用

页码：1097 / 1100

页数：4

共 6 条

[1] GENERAL METHOD FOR ISOLATION OF HIGH MOLECULAR-WEIGHT DNA FROM EUKARYOTES [J].