ELDA: Extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays

被引：1577

作者：

Hu, Yifang ^{[1
]}

Smyth, Gordon K. ^{[1
]}

机构：

[1] Walter & Eliza Hall Inst Med Res, Bioinformat Div, Parkville, Vic 3052, Australia

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2009年 / 347卷 / 1-2期

关键词：

Stem cell assays; Single-hit hypothesis; Generalized linear models; Clopper-Pearson; Likelihood ratio tests; Computer software; HIT POISSON MODEL; GENERALIZED LINEAR-MODELS; BONE-MARROW; CANCER; DIFFERENTIATION; GENERATION; IDENTIFICATION; FREQUENCIES; TESTS; BLOOD;

D O I：

10.1016/j.jim.2009.06.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

ELDA is a software application for limiting dilution analysis (LIDA), with particular attention to the needs of stem cell assays. It is the first limiting dilution analysis software to provide meaningful confidence intervals for all LIDA data sets, including those with 0% or 100% responses. Other features include a test of the adequacy of the single-hit hypothesis, tests for frequency differences between multiple data sets, and the ability to take advantage of cases where the number of cells in the sample is counted exactly. A webtool at http://bioinf.wehi.edu.au/software/elda/ provides an easy user interface. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：70 / 78

页数：9

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