The diagnostic substrate bicyclohexane reveals a radical mechanism for bacterial cytochrome P450 in whole cells

被引：26

作者：

Austin, Rachel N. ^{[1
]}

Deng, Dayi

Jiang, Yongying

Luddy, Kate

van Beilen, Jan B.

de Montellano, Paul R. Ortiz

Groves, John T.

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[2] Bates Coll, Dept Chem, Lewiston, ME 04240 USA

[3] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

[4] ETH, Inst Biotechnol, CH-8093 Zurich, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 48期

关键词：

alkanes; cytochromes; oxidation; radical ions; reaction mechanisms;

D O I：

10.1002/anie.200603282

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) On home ground: The reaction mechanisms of bacterial alkane-oxidizing cytochrome P450s were determined in their native environment using a novel diagnostic substrate probe, bicyclohexane, in whole cells and cell-free extracts (see picture). Purified P450cam also oxidizes bicyclohexane. Clear evidence for a substrate-based radical with a lifetime of 75-250 ps was obtained. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：8192 / 8194

页数：3

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