Fasciola hepatica:: Heterologous expression and functional characterization of a thioredoxin peroxidase

被引：34

作者：

Salazar-Calderón, M ^{[1
]}

Martín-Alonso, JM ^{[1
]}

de Eguino, ADR ^{[1
]}

Casais, R ^{[1
]}

Marín, MS ^{[1
]}

Parra, F ^{[1
]}

机构：

[1] Univ Oviedo, Inst Univ Biotecnol Asturias, CSIC, Dept Bioquim & Biol Mol, E-33006 Oviedo, Spain

来源：

EXPERIMENTAL PARASITOLOGY | 2000年 / 95卷 / 01期

关键词：

Fasciola hepatica; antioxidant protein; TSA protein;

D O I：

10.1006/expr.2000.4495

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A Fasciola hepatica cDNA clone of 779 bp was isolated from an adult worm cDNA expression library by immunological screening using a rabbit serum against the excretory-secretory antigens. The nucleotide sequence of the cDNA revealed the presence of an open reading frame of 582 bp which encoded a 194-amino-acid-residue polypeptide (M-r 21,723 Da) showing a high degree of homology to thioredoxin peroxidases. This putative antioxidant protein gene was expressed in Escherichia coli as a GST fusion protein. The recombinant fusion protein showed in vitro antioxidant properties and protected rabbit muscle enolase and E. coli glutamine synthetase from inactivation by nonenzymatic Fe3+/O-2/DTT or Fe3+/O-2/ascorbate metal-catalyzed oxidation systems. (C) 2000 Academic Press.

引用

页码：63 / 70

页数：8

共 29 条

[1] IN-VITRO EFFECTS OF FASCIOLA-HEPATICA ON THE MAIN FUNCTIONS OF POLYMORPHONUCLEAR LEUKOCYTES - CHEMOTAXIS AND FREE-RADICAL GENERATION INDUCED BY PHAGOCYTOSIS [J].