Microbial sensors for small molecules: Development of a mevalonate biosensor

被引：65

作者：

Pfleger, Brian F.

Pitera, Douglas J.

Newman, Jack D.

Martin, Vincent J. J.

Keasling, Jay D.

机构：

[1] Univ Calif Berkeley, Berkeley Ctr Synthet Biol, Dept Chem Engn, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Lawrence Berkeley Lab, Synthet Biol Dept, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

METABOLIC ENGINEERING | 2007年 / 9卷 / 01期

基金：

美国国家科学基金会;

关键词：

mevalonate; biosensor; DXP pathway; isoprenoid; mevalonate pathway; high-throughput screening;

D O I：

10.1016/j.ymben.2006.08.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We describe a novel biosensor strain for detection and quantification of a small molecule, mevalonate. The biosensor strain is an Escherichia coli mevalonate auxotroph that expresses the green fluorescent protein and reports on the mevalonate concentration in the growth medium through a change in growth rate. A model describing the growth rate dependence on mevalonate was developed in order to use the biosensor strain for high-throughput screening (HTS) and quantitative measurement of mevalonate in the extracellular environment. In general, this method should be applicable to the quantification of any small molecule for which an auxotroph can be developed and will be useful for HTS of evolved metabolic pathways for which there is no readily available screen or selection. (C) 2006 Elsevier Inc. All rights reserved.

引用

页码：30 / 38

页数：9

共 23 条

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