In vitro antioxidant and, α-glucosidase inhibitory activities and comprehensive metabolite profiling of methanol extract and its fractions from Clinacanthus nutans

被引：39

作者：

Alam, Md. Ariful ^{[1
]}

Zaidul, I. S. M. ^{[1
]}

Ghafoor, Kashif ^{[2
]}

Sahena, F. ^{[3
]}

Hakim, M. A. ^{[4
]}

Rafii, M. Y. ^{[4
]}

Abir, H. M. ^{[5
]}

Bostanudin, M. F. ^{[6
]}

Perumal, V. ^{[1
]}

Khatib, A. ^{[1
]}

机构：

[1] IIUM, Fac Pharm, Kuantan Campus, Kuantan 25200, Pahang, Malaysia

[2] King Saud Univ, Dept Food Sci & Nutr, Riyadh 11451, Saudi Arabia

[3] IIUM, Fac Sci, Kuantan Campus, Kuantan 25200, Pahang, Malaysia

[4] Univ Putra Malaysia, Inst Trop Agr, Serdang 43400, Selangor Darul, Malaysia

[5] Bangladesh Agr Univ, Fac Agr Engn & Technol, Mymensingh, Bangladesh

[6] Cyberjaya Univ Coll Med Sci, Dept Pharmaceut Technol & Ind, Fac Pharm, 3410,Jalan Teknokrat 3,Cyber 4, Cyberjaya 63000, Selangor Darul, Malaysia

来源：

BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2017年 / 17卷

关键词：

Clinacanthus nutans; Antioxidant activity; alpha-glucosidase inhibitory activity; Reducing power; Total phenolic; Total flavonoid; Activity phytochemical profiling; PHENOLIC-COMPOUNDS; AMYLASE; PLANTS; ENZYMES; HERBS;

D O I：

10.1186/s12906-017-1684-5

中图分类号：

R [医药、卫生];

学科分类号：

100218 [急诊医学];

摘要：

Background: This study was aimed to evaluate antioxidant and alpha-glucosidase inhibitory activity, with a subsequent analysis of total phenolic and total flavonoid content of methanol extract and its derived fractions from Clinacanthus nutans accompanied by comprehensive phytochemical profiling. Methods: Liquid-liquid partition chromatography was used to separate methanolic extract to get hexane, ethyl acetate, butanol and residual aqueous fractions. The total antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazy (DPPH) radical scavenging and ferric reducing antioxidant power assay (FRAP). The antidiabetic activity of methanol extract and its consequent fractions were examined by alpha-glucosidase inhibitory bioassay. The chemical profiling was carried out by gas chromatography coupled with quadrupole time-of-flight mass spectrometry (GC Q-TOF MS). Results: The total yield for methanol extraction was (12.63 +/- 0.98) % (w/w) and highest fractionated value found for residual aqueous (52.25 +/- 1.01) % (w/w) as compared to the other fractions. Significant DPPH free radical scavenging activity was found for methanolic extract (63.07 +/- 0.11) % and (79.98 +/- 0.31) % for ethyl acetate fraction among all the fractions evaluated. Methanol extract was the most prominent in case of FRAP (141.89 +/- 0.87 mu g AAE/g) whereas most effective reducing power observed in ethyl acetate fraction (133.6 +/- 0.2987 mu g AAE/g). The results also indicated a substantial alpha-glucosidase inhibitory activity for butanol fraction (72.16 +/- 1.0) % and ethyl acetate fraction (70.76 +/- 0.49) %. The statistical analysis revealed that total phenolic and total flavonoid content of the samples had the significant (p < 0.05) impact on DPPH free radical scavenging and alpha-glucosidase inhibitory activity. Conclusion: Current results proposed the therapeutic potential of Clinacanthus nutans, especially ethyl acetate and butanol fraction as chemotherapeutic agent against oxidative related cellular damages and control the postprandial hyperglycemia. The phytochemical investigation showed the existence of active constituents in Clinacanthus nutans extract and fractions.

引用

页数：10

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