Expression of antioxidant enzymes in diseases of the human pancreas: Another link between chronic pancreatitis and pancreatic cancer

被引:107
作者
Cullen, JJ
Mitros, FA
Oberley, LW
机构
[1] Univ Iowa, Coll Med, Dept Surg, Iowa City, IA 52242 USA
[2] Univ Iowa, Coll Med, Dept Pathol, Iowa City, IA 52242 USA
[3] Univ Iowa, Coll Med, Dept Radiat Oncol, Iowa City, IA 52242 USA
关键词
pancreatic cancer; superoxide dismutase; pancreatitis; catalase; glutathione peroxidase;
D O I
10.1097/00006676-200301000-00005
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Introduction: Chronic pancreatitis is a significant risk factor for pancreatic cancer and is associated with the generation of reactive oxygen species. Cells contain a large number of antioxidants to prevent or repair the damage caused by reactive oxygen species. There are three major types of primary intracellular antioxidant enzymes in mammalian cells: superoxide dismutase (SOD), catalase, and peroxidase, of which glutathione peroxidase is the most prominent. Aim: To determine the level of antioxidant enzymes in human pancreas from normal, chronic pancreatitis, and pancreatic cancer specimens. Methodology: Immunohistochemical analysis for manganese SOD, copper/zinc SOD, catalase, and glutathione peroxidase expression using the avidin-biotin-peroxidase complex method was performed on pancreatic specimens previously fixed in formalin and embedded in paraffin. A quantitative digital imaging methodology was used to examine antioxidant staining in the pancreatic tissue. Cytoplasmic regions of ductal and acinar cells were identified and digitized. Mean gray-level pixel values were then obtained for each of these regions. Results: Cytoplasmic values of manganese SOD, catalase, and glutathione peroxidase were decreased in pancreatic cells from chronic pancreatitis specimens when compared with normal pancreas. In pancreatic carcinoma specimens, mean cytoplasmic gray-level values of all antioxidant enzymes were decreased when compared with normal pancreas. Conclusion: There appears to be a gradual decrease in antioxidant enzyme expression in pancreatic cells from normal pancreas to chronic pancreatitis to pancreatic cancer.
引用
收藏
页码:23 / 27
页数:5
相关论文
共 25 条
[1]   HIGH-INCIDENCE OF EXTRA-PANCREATIC CARCINOMA IN CHRONIC-PANCREATITIS [J].
AMMANN, RW ;
KNOBLAUCH, M ;
MOHR, P ;
DEYHLE, P ;
LARGIADER, F ;
AKOVBIANTZ, A ;
SCHULER, G ;
SCHNEIDER, J .
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY, 1980, 15 (04) :395-399
[2]  
Baker AM, 1997, PROSTATE, V32, P229, DOI 10.1002/(SICI)1097-0045(19970901)32:4<229::AID-PROS1>3.0.CO
[3]  
2-E
[4]   THE ROLE OF THE CELLULAR ANTIOXIDANT DEFENSE IN OXIDANT CARCINOGENESIS [J].
CERUTTI, P ;
GHOSH, R ;
OYA, Y ;
AMSTAD, P .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1994, 102 :123-129
[5]  
CERUTTI PA, 1985, SCIENCE, V227, P7634
[6]   Mitogenic signaling mediated by oxidants in ras-transformed fibroblasts [J].
Irani, K ;
Xia, Y ;
Zweier, JL ;
Sollott, SJ ;
Der, CJ ;
Fearon, ER ;
Sundaresan, M ;
Finkel, T ;
GoldschmidtClermont, PJ .
SCIENCE, 1997, 275 (5306) :1649-1652
[7]   Cancer statistics, 2002 [J].
Jemal, A ;
Thomas, A ;
Murray, T ;
Thun, M .
CA-A CANCER JOURNAL FOR CLINICIANS, 2002, 52 (01) :23-47
[8]   Immunolocalization and adenoviral vector-mediated manganese superoxide dismutase gene transfer to experimental oral tumors [J].
Lam, EWN ;
Hammad, HM ;
Zwacka, R ;
Darby, CJ ;
Baumgardner, KR ;
Davidson, BL ;
Oberley, TD ;
Engelhardt, JF ;
Oberley, LW .
JOURNAL OF DENTAL RESEARCH, 2000, 79 (06) :1410-1417
[9]   PANCREATITIS AND THE RISK OF PANCREATIC-CANCER [J].
LOWENFELS, AB ;
MAISONNEUVE, P ;
CAVALLINI, G ;
AMMANN, RW ;
LANKISCH, PG ;
ANDERSEN, JR ;
DIMAGNO, EP ;
ANDRENSANDBERG, A ;
DOMELLOF, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1993, 328 (20) :1433-1437
[10]   DISTINGUISHING PANCREATIC-CARCINOMA FROM OTHER PERIAMPULLARY CARCINOMAS BY ANALYSIS OF MUTATIONS IN THE KIRSTEN-RAS ONCOGENE [J].
MOTOJIMA, K ;
TSUNODA, T ;
KANEMATSU, T ;
NAGATA, Y ;
URANO, T ;
SHIKU, H .
ANNALS OF SURGERY, 1991, 214 (06) :657-662