Effect of nanometer hydroxyapatite particles on rat macrophage at mRNA level

被引：3

作者：

Ding, Tingting ^{[1
]}

Sun, Jiao ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Peoples Hosp 9, Sch Med, Shanghai Biomat Res & Testing Ctr, Shanghai 200023, Peoples R China

来源：

BIOCERAMICS, VOL 19, PTS 1 AND 2 | 2007年 / 330-332卷

基金：

中国国家自然科学基金;

关键词：

nanometer hydroxyapatite particles; RT-PCR; DNA damage;

D O I：

10.4028/www.scientific.net/KEM.330-332.287

中图分类号：

TQ174 [陶瓷工业]; TB3 [工程材料学];

学科分类号：

0805 [材料科学与工程]; 080502 [材料学];

摘要：

To investigate the potential cytotoxicity of nanometre hydroxyapatite (nano-HA), the transcription and expression of genes about DNA damage were determined by semi-quantitative RT-PCR. The results showed that 100 mu g/ml nano-HA can induce P53, P21 and HSP70 up-regulated, and these genes have obviously difference compared with negative control (p < 0.05). Furthermore, their expressions were depending on the concentration of particles. But the influence of nano-HA on Gadd45 was not visible. Above results indicate that cytotoxicity of nano-HA particles on macrophage would be determined by these genes at mRNA level, which can regulate cell cycle and DNA damage. Moreover, P53 and HSP70 would be ideal indictors to evaluate the biological safety of nanometer ceramics particles.

引用

页码：287 / +

页数：2

共 7 条

[1]

p53-independent regulation of p21Waf1/Cip1 expression and senescence by Chk2 [J].